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Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation

Received: 17 May 2023     Accepted: 5 June 2023     Published: 27 June 2023
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Abstract

DNA methylation is an epigenetic mechanism involved in the regulation of blood lipid levels which contribute to the cardiovascular risk in diabetic patients. Active agents that target atherogenic dyslipidemias epigenetically are therefore of paramount interest for prevention of cardiovascular complications in these patients. This study aims at evaluating the antiatherogenic effect of hydroethanolic extract of A. congolensis (HEEAC) on diabetic rats. Diabetes was induced by a single dose of 50 mg/kg b.w streptozotocin. Diabetic rats were then treated with 150 mg/kg b.w of HEEAC or with atorvastatin 10 mg/kg b.w (reference drug) for 28 days. At the end of the experimental period, rats were sacrificed, blood samples and liver tissues were collected and the plasma concentrations of triglycerides (TG), total cholesterol (TC), HDL-cholesterol and LDL-cholesterol were assessed. The atherogenic indices were also calculated and the liver and blood DNA extracted to determine DNA methylation. Comparing to untreated diabetic rats, the HEEAC treated group showed significant lower values of TG 245.98 ± 41.39 vs 57.88 ± 10.64 mg/dL (p < 0.05), TC 159.88 ± 17.56 vs. 87.77 ± 9.51 mg/dL (p < 0.05), LDL-C 94.51 ± 0.66 vs 48.71 ± 1.45 mg/dL (p < 0.05) and higher values of HDL-C 19.55 ± 1.6 vs 27.49 ± 1.45 (p < 0.05). Atherogenic indices were significantly lowered in HEEAC-group. The effects of HEEAC on lipid profile and atherogenic indices were significantly higher than atorvastatin. Also, the percentage of global hepatic DNA methylation (0.34±0.033 vs 0.63±0.023 %) was significantly increased in HEEAC-group compared to untreated diabetic group. DNA methylation profile in HEEAC-group correlated negatively and significantly (P < 0.01) with LDL-C and TC levels. HEEAC prevents atherogenic dyslipidemia in diabetic rats by targeting global DNA-methylation status in diabetic rats.

Published in American Journal of Biomedical and Life Sciences (Volume 11, Issue 3)
DOI 10.11648/j.ajbls.20231103.14
Page(s) 53-59
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Diabetes, DNA Methylation, Liver, Lipid Profile, Atherogenic Risk, Autranella congolensis

References
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Cite This Article
  • APA Style

    Maboune, N., Fonkoua, M., Goda, D., Ngoumen, D. J. N., Nanhah, J. K., et al. (2023). Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation. American Journal of Biomedical and Life Sciences, 11(3), 53-59. https://doi.org/10.11648/j.ajbls.20231103.14

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    ACS Style

    Maboune, N.; Fonkoua, M.; Goda, D.; Ngoumen, D. J. N.; Nanhah, J. K., et al. Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation. Am. J. Biomed. Life Sci. 2023, 11(3), 53-59. doi: 10.11648/j.ajbls.20231103.14

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    AMA Style

    Maboune N, Fonkoua M, Goda D, Ngoumen DJN, Nanhah JK, et al. Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation. Am J Biomed Life Sci. 2023;11(3):53-59. doi: 10.11648/j.ajbls.20231103.14

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  • @article{10.11648/j.ajbls.20231103.14,
      author = {Nafissatou Maboune and Martin Fonkoua and David Goda and Dany Joël Ngassa Ngoumen and Jules Kamga Nanhah and Bruno Dupon Ambamba Akamba and Jean Paul Chedjou and Maxwell Wandji Nguedjo and Fils Armand Ella and Javeres Leonel Ntepe Mbah and Guy Roussel Takuissu Nguemto and Judith Laure Ngondi},
      title = {Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {11},
      number = {3},
      pages = {53-59},
      doi = {10.11648/j.ajbls.20231103.14},
      url = {https://doi.org/10.11648/j.ajbls.20231103.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20231103.14},
      abstract = {DNA methylation is an epigenetic mechanism involved in the regulation of blood lipid levels which contribute to the cardiovascular risk in diabetic patients. Active agents that target atherogenic dyslipidemias epigenetically are therefore of paramount interest for prevention of cardiovascular complications in these patients. This study aims at evaluating the antiatherogenic effect of hydroethanolic extract of A. congolensis (HEEAC) on diabetic rats. Diabetes was induced by a single dose of 50 mg/kg b.w streptozotocin. Diabetic rats were then treated with 150 mg/kg b.w of HEEAC or with atorvastatin 10 mg/kg b.w (reference drug) for 28 days. At the end of the experimental period, rats were sacrificed, blood samples and liver tissues were collected and the plasma concentrations of triglycerides (TG), total cholesterol (TC), HDL-cholesterol and LDL-cholesterol were assessed. The atherogenic indices were also calculated and the liver and blood DNA extracted to determine DNA methylation. Comparing to untreated diabetic rats, the HEEAC treated group showed significant lower values of TG 245.98 ± 41.39 vs 57.88 ± 10.64 mg/dL (p < 0.05), TC 159.88 ± 17.56 vs. 87.77 ± 9.51 mg/dL (p < 0.05), LDL-C 94.51 ± 0.66 vs 48.71 ± 1.45 mg/dL (p < 0.05) and higher values of HDL-C 19.55 ± 1.6 vs 27.49 ± 1.45 (p < 0.05). Atherogenic indices were significantly lowered in HEEAC-group. The effects of HEEAC on lipid profile and atherogenic indices were significantly higher than atorvastatin. Also, the percentage of global hepatic DNA methylation (0.34±0.033 vs 0.63±0.023 %) was significantly increased in HEEAC-group compared to untreated diabetic group. DNA methylation profile in HEEAC-group correlated negatively and significantly (P < 0.01) with LDL-C and TC levels. HEEAC prevents atherogenic dyslipidemia in diabetic rats by targeting global DNA-methylation status in diabetic rats.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation
    AU  - Nafissatou Maboune
    AU  - Martin Fonkoua
    AU  - David Goda
    AU  - Dany Joël Ngassa Ngoumen
    AU  - Jules Kamga Nanhah
    AU  - Bruno Dupon Ambamba Akamba
    AU  - Jean Paul Chedjou
    AU  - Maxwell Wandji Nguedjo
    AU  - Fils Armand Ella
    AU  - Javeres Leonel Ntepe Mbah
    AU  - Guy Roussel Takuissu Nguemto
    AU  - Judith Laure Ngondi
    Y1  - 2023/06/27
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ajbls.20231103.14
    DO  - 10.11648/j.ajbls.20231103.14
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 53
    EP  - 59
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20231103.14
    AB  - DNA methylation is an epigenetic mechanism involved in the regulation of blood lipid levels which contribute to the cardiovascular risk in diabetic patients. Active agents that target atherogenic dyslipidemias epigenetically are therefore of paramount interest for prevention of cardiovascular complications in these patients. This study aims at evaluating the antiatherogenic effect of hydroethanolic extract of A. congolensis (HEEAC) on diabetic rats. Diabetes was induced by a single dose of 50 mg/kg b.w streptozotocin. Diabetic rats were then treated with 150 mg/kg b.w of HEEAC or with atorvastatin 10 mg/kg b.w (reference drug) for 28 days. At the end of the experimental period, rats were sacrificed, blood samples and liver tissues were collected and the plasma concentrations of triglycerides (TG), total cholesterol (TC), HDL-cholesterol and LDL-cholesterol were assessed. The atherogenic indices were also calculated and the liver and blood DNA extracted to determine DNA methylation. Comparing to untreated diabetic rats, the HEEAC treated group showed significant lower values of TG 245.98 ± 41.39 vs 57.88 ± 10.64 mg/dL (p < 0.05), TC 159.88 ± 17.56 vs. 87.77 ± 9.51 mg/dL (p < 0.05), LDL-C 94.51 ± 0.66 vs 48.71 ± 1.45 mg/dL (p < 0.05) and higher values of HDL-C 19.55 ± 1.6 vs 27.49 ± 1.45 (p < 0.05). Atherogenic indices were significantly lowered in HEEAC-group. The effects of HEEAC on lipid profile and atherogenic indices were significantly higher than atorvastatin. Also, the percentage of global hepatic DNA methylation (0.34±0.033 vs 0.63±0.023 %) was significantly increased in HEEAC-group compared to untreated diabetic group. DNA methylation profile in HEEAC-group correlated negatively and significantly (P < 0.01) with LDL-C and TC levels. HEEAC prevents atherogenic dyslipidemia in diabetic rats by targeting global DNA-methylation status in diabetic rats.
    VL  - 11
    IS  - 3
    ER  - 

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Author Information
  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon; Center of Nutrition and Functional Foods, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Buea, Buea, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon; Centre for Food, Food Security and Nutrition Research, Institute of Medical Research and Medicinal Plant Studies, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon; Center of Nutrition and Functional Foods, Yaounde, Cameroon; Department of Pharmacology, Faculty of Health Sciences, School of Clinical Medicines, University of Fee State, Bloemfontein, South Africa

  • Laboratory of Human Metabolism and Non-Communicable Disease, Institute of Medical Research and Medicinal Plant Studies, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon; Centre for Food, Food Security and Nutrition Research, Institute of Medical Research and Medicinal Plant Studies, Yaounde, Cameroon

  • Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon

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